Agrobacterium-Mediated Transformation

This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. Agrobacterium-mediated transformation is a widely used method for generating transgenic plants. Key steps include the removal of the T-DNA plasmid from Agrobacterium and cleavage using a restriction enzyme. The desired foreign DNA, cut with the same enzyme, is inserted into the plasmid. The recombinant Ti plasmid is re-introduced into the bacterium, which then transfers the T-DNA carrying the inserted gene into a plant cell chromosome. Culturing these transformed plant cells later results in a transgenic plant expressing the integrated foreign gene. [Adapted from GPT4, 2023]

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

Yes, BioRender icons and templates meet journal, grant, and copyright standards for publication, and many researchers cite BioRender directly in their figure legends.

Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

Researchers and scientists use this template for publications, funding proposals, grant applications, presentations, posters, and more.