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Antibody-Dependent Cellular Cytotoxicity Method Against Spike Expressing Cells

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Andrés Finzi, PhD

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Description: Fc-mediated effector functions, including ADCC, are critical in SARS-CoV-2 immune responses. Using a GFP-tagged SARS-CoV-2 Spike-expressing cell line, this protocol measures ADCC activity. Fluorescence reduction reflects cell death induced by anti-Spike monoclonal antibodies or plasma from infected or vaccinated individuals, providing an optimized method to evaluate ADCC and antibody functionality against SARS-CoV-2.

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Fc-mediated effector functions, including ADCC, are critical in SARS-CoV-2 immune responses. Using a GFP-tagged SARS-CoV-2 Spike-expressing cell line, this protocol measures ADCC activity. Fluorescence reduction reflects cell death induced by anti-Spike monoclonal antibodies or plasma from infected or vaccinated individuals, providing an optimized method to evaluate ADCC and antibody functionality against SARS-CoV-2.

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