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Isoelectric Focusing (IEF)

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Sally Kim
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Description: This template shows isoelectric focusing (IEF), illustrating how a protein migrates through a pH gradient based on its charge until it reaches its isoelectric point, where it carries no net charge and stops migrating. It shows three sequential panels tracking positively and negatively charged proteins moving toward pH 6.5, where different proteins settle at different positions based on their distinct isoelectric points, allowing separation.

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What does the Isoelectric Focusing (IEF) template show?

This template shows isoelectric focusing (IEF), illustrating how a protein migrates through a pH gradient based on its charge until it reaches its isoelectric point, where it carries no net charge and stops migrating. It shows three sequential panels tracking positively and negatively charged proteins moving toward pH 6.5, where different proteins settle at different positions based on their distinct isoelectric points, allowing separation.

Is the Isoelectric Focusing (IEF) template free to use?

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Can I edit or customize this template?

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

Is this template accepted for use in journals, theses, or grant applications?

Yes, BioRender icons and templates meet journal, grant, and copyright standards for publication, and many researchers cite BioRender directly in their figure legends.

How do I cite BioRender if I use this template?

Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

What is Isoelectric Focusing (IEF) used for / who typically uses this template?

Researchers and scientists use this template for publications, funding proposals, grant applications, presentations, posters, and more.

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