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Measurement of mRNA Expression Knockdown Using siRNA Through RT-qPCR

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Ioanna Gianopoulos

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Description: This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. This diagram highlights the general workflow for validating siRNA knockdown efficiency for a target gene and determining mRNA expression for other genes of interest. First, siRNA is transfected into cells and incubated for 48-72 hours for efficient knockdown. Next, RNA is either isolated following reverse transcription to cDNA, or stored at -80 °C for future use. Lastly, the cDNA is amplified through qPCR and mRNA is assessed through corresponding Ct values.

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This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. This diagram highlights the general workflow for validating siRNA knockdown efficiency for a target gene and determining mRNA expression for other genes of interest. First, siRNA is transfected into cells and incubated for 48-72 hours for efficient knockdown. Next, RNA is either isolated following reverse transcription to cDNA, or stored at -80 °C for future use. Lastly, the cDNA is amplified through qPCR and mRNA is assessed through corresponding Ct values.

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