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Sandwich ELISA

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Samara Ona
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Description: This figure template "Sandwich ELISA" is assembled using dynamic BioRender assets (icons, lines, shapes and/or text) and is fully editable. To customize your own personalized figure using this template as a starting point, click "Use Template". Sandwich ELISA is an immunoassay technique used to detect and quantify antigens or antibodies in a sample. This method involves immobilizing a capture antibody on the surface of a microtiter plate, which specifically binds to the antigen of interest. A primary antibody is then added to the well, which binds to another epitope on the antigen and creates a "sandwich" between two antibodies. A detection antibody conjugated to biotin is then added to bind the primary antibody. Streptavidin-HRPO then binds biotin, and catalyzes a chromogenic substrate to produce a signal that is proportional to the concentration of the antigen. [Template description adapted from GPT3.5, 2023]

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BioRender-Vorlagen-FAQs

What does the Sandwich ELISA template show?

This figure template "Sandwich ELISA" is assembled using dynamic BioRender assets (icons, lines, shapes and/or text) and is fully editable. To customize your own personalized figure using this template as a starting point, click "Use Template". Sandwich ELISA is an immunoassay technique used to detect and quantify antigens or antibodies in a sample. This method involves immobilizing a capture antibody on the surface of a microtiter plate, which specifically binds to the antigen of interest. A primary antibody is then added to the well, which binds to another epitope on the antigen and creates a "sandwich" between two antibodies. A detection antibody conjugated to biotin is then added to bind the primary antibody. Streptavidin-HRPO then binds biotin, and catalyzes a chromogenic substrate to produce a signal that is proportional to the concentration of the antigen. [Template description adapted from GPT3.5, 2023]

Is the Sandwich ELISA template free to use?

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Can I edit or customize this template?

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

Is this template accepted for use in journals, theses, or grant applications?

Yes, BioRender icons and templates meet journal, grant, and copyright standards for publication, and many researchers cite BioRender directly in their figure legends.

How do I cite BioRender if I use this template?

Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

What is Sandwich ELISA used for / who typically uses this template?

Researchers and scientists use this template for publications, funding proposals, grant applications, presentations, posters, and more.

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