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Trophoblast organoids grown with physiologic polarity

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Carolyn Coyne, PhD

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Description: Depiction of the culturing of trophoblast organoids (TOs) containing an inward-facing syncytiotrophoblast (STB) in Matrigel. TOs are released from Matrigel and grown in suspension for ~48hrs, which generates TOs with a physiolocially relevant STB layer on the outer surface of the organoid. Schematic of STBin (left) or STBout (right) TOs representing the cellular orientation of cytotrophoblasts (CTBs, in green), extravillous trophoblasts (in blue), and the syncytiotrophoblast (in red).

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Depiction of the culturing of trophoblast organoids (TOs) containing an inward-facing syncytiotrophoblast (STB) in Matrigel. TOs are released from Matrigel and grown in suspension for ~48hrs, which generates TOs with a physiolocially relevant STB layer on the outer surface of the organoid. Schematic of STBin (left) or STBout (right) TOs representing the cellular orientation of cytotrophoblasts (CTBs, in green), extravillous trophoblasts (in blue), and the syncytiotrophoblast (in red).

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