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Experimental Protocol for Differentiation and Transfection of 3T3-L1 Cells

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Amina Tucak-Smajić

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Description: The figure describes the experimental protocol for differentiating 3T3-L1 preadipocytes into mature adipocytes. The protocol consists of several steps: 1. Seeding the cells into 96-well plates 24h prior to transfection 2. Transfection of cells with DDS 3. Addition of induction medium to cells 24h after transfection to trigger the differentiation 4. Addition of differentiation medium on day 2 and day 4 5. ORO staining of lipid droplets in mature adipocytes

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The figure describes the experimental protocol for differentiating 3T3-L1 preadipocytes into mature adipocytes. The protocol consists of several steps: 1. Seeding the cells into 96-well plates 24h prior to transfection 2. Transfection of cells with DDS 3. Addition of induction medium to cells 24h after transfection to trigger the differentiation 4. Addition of differentiation medium on day 2 and day 4 5. ORO staining of lipid droplets in mature adipocytes

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