Generate Human Monocytic Dendritic Cells From Peripheral Blood

This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. To generate human monocytic dendritic cells, peripheral blood is collected from healthy donors, layered on top of a density gradient cell separation medium, and centrifuged. White blood cells are collected and washed with PBS before counting peripheral blood mononuclear cells (PBMCs). Subsequently, CD14+ monocytes are isolated using magnetic separation and plated in a 6-well plate with 800 U/mL GM-CSF and 400 U/mL IL-4. Media is replenished as needed. Immature human monocytic-DCs can be collected on day 5 of culture.

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