Modèle BioRender

Generate Human Monocytic Dendritic Cells From Peripheral Blood

Créé par

Megan Stanley Molina

•

BioRender Team

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Description: This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. To generate human monocytic dendritic cells, peripheral blood is collected from healthy donors, layered on top of a density gradient cell separation medium, and centrifuged. White blood cells are collected and washed with PBS before counting peripheral blood mononuclear cells (PBMCs). Subsequently, CD14+ monocytes are isolated using magnetic separation and plated in a 6-well plate with 800 U/mL GM-CSF and 400 U/mL IL-4. Media is replenished as needed. Immature human monocytic-DCs can be collected on day 5 of culture.

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What does the Generate Human Monocytic Dendritic Cells From Peripheral Blood template show?

This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication. To generate human monocytic dendritic cells, peripheral blood is collected from healthy donors, layered on top of a density gradient cell separation medium, and centrifuged. White blood cells are collected and washed with PBS before counting peripheral blood mononuclear cells (PBMCs). Subsequently, CD14+ monocytes are isolated using magnetic separation and plated in a 6-well plate with 800 U/mL GM-CSF and 400 U/mL IL-4. Media is replenished as needed. Immature human monocytic-DCs can be collected on day 5 of culture.

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Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

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Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

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