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Dual FluoroSpot Assay Protocol

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Mina Nashed

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BioRender Team

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Description: This template shows a five-step dual FluoroSpot assay protocol in which stimulated cells secrete two antigens that are captured by immobilized antibodies in a well. TAG-labeled and biotinylated detection antibodies bind the two analytes, followed by fluorophore-labeled anti-TAG antibody and streptavidin-fluorophore conjugates, producing pink and blue fluorescent spots at the well base that are quantified separately for each antigen.

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What does the Dual FluoroSpot Assay Protocol template show?

This template shows a five-step dual FluoroSpot assay protocol in which stimulated cells secrete two antigens that are captured by immobilized antibodies in a well. TAG-labeled and biotinylated detection antibodies bind the two analytes, followed by fluorophore-labeled anti-TAG antibody and streptavidin-fluorophore conjugates, producing pink and blue fluorescent spots at the well base that are quantified separately for each antigen.

Is the Dual FluoroSpot Assay Protocol template free to use?

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What is Dual FluoroSpot Assay Protocol used for / who typically uses this template?

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