Modèle BioRender

Gibson Assembly Cloning 1

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Créé par 
Samara Ona
  •  
BioRender Team
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Description: Gibson Assembly Protocol is a powerful molecular cloning technique, enabling the seamless construction of recombinant DNA molecules. The process starts with linear vector and DNA inserts featuring overlapping ends. Using Gibson assembly, these fragments are simultaneously and seamlessly joined in a single isothermal reaction. The resulting transformed vector is then introduced into competent bacterial cells through transformation. This method simplifies the assembly of multiple DNA fragments into a single, functional recombinant molecule without relying on restriction enzymes or ligases. [Adapted from GPT4, 2023]

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What does the Gibson Assembly Cloning 1 template show?

Gibson Assembly Protocol is a powerful molecular cloning technique, enabling the seamless construction of recombinant DNA molecules. The process starts with linear vector and DNA inserts featuring overlapping ends. Using Gibson assembly, these fragments are simultaneously and seamlessly joined in a single isothermal reaction. The resulting transformed vector is then introduced into competent bacterial cells through transformation. This method simplifies the assembly of multiple DNA fragments into a single, functional recombinant molecule without relying on restriction enzymes or ligases. [Adapted from GPT4, 2023]

Is the Gibson Assembly Cloning 1 template free to use?

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Can I edit or customize this template?

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

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Yes, BioRender icons and templates meet journal, grant, and copyright standards for publication, and many researchers cite BioRender directly in their figure legends.

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Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

What is Gibson Assembly Cloning 1 used for / who typically uses this template?

Researchers and scientists use this template for publications, funding proposals, grant applications, presentations, posters, and more.

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