Modèle BioRender

Two-tailed qPCR

Créé par

Martina Maritan

•

BioRender Team

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Description: This template shows the four-step two-tailed qPCR method for detecting miRNA: a two-tailed RT primer with 5' and 3' hemiprobes binds cooperatively at each end of the target miRNA, reverse transcriptase elongates the primer into cDNA, and the cDNA is then amplified by qPCR using two target-specific primers across two cycles.

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FAQ sur les modèles BioRender

What does the Two-tailed qPCR template show?

This template shows the four-step two-tailed qPCR method for detecting miRNA: a two-tailed RT primer with 5' and 3' hemiprobes binds cooperatively at each end of the target miRNA, reverse transcriptase elongates the primer into cDNA, and the cDNA is then amplified by qPCR using two target-specific primers across two cycles.

Is the Two-tailed qPCR template free to use?

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Can I edit or customize this template?

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

Is this template accepted for use in journals, theses, or grant applications?

Yes, BioRender icons and templates meet journal, grant, and copyright standards for publication, and many researchers cite BioRender directly in their figure legends.

How do I cite BioRender if I use this template?

Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

What is Two-tailed qPCR used for / who typically uses this template?

Researchers and scientists use this template for publications, funding proposals, grant applications, presentations, posters, and more.