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BioRender Template
Two-tailed qPCR
Description
This template shows the four-step two-tailed qPCR method for detecting miRNA: a two-tailed RT primer with 5' and 3' hemiprobes binds cooperatively at each end of the target miRNA, reverse transcriptase elongates the primer into cDNA, and the cDNA is then amplified by qPCR using two target-specific primers across two cycles.
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