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Comparison of TCID50 and Plaque Assays for Defining the Infectivity of a Virus Sample

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Stephen Polyak

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Description: The figure shows the methodological differences in viral TCID50 and Plaque assays. TCID50 requires mathematical transformations of the data to calculate the final titer, using one of several methods such as: Spearman–Karber, Reed–Muench, Improved Karber , Weil, or Probit/logit regression. For plaque assay, the math is simpler: (# of plaques X dilution factor) divided by the volume of the inoculum added to cells in milliliters = plaque forming units per ml.

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What does the Comparison of TCID50 and Plaque Assays for Defining the Infectivity of a Virus Sample template show?

The figure shows the methodological differences in viral TCID50 and Plaque assays. TCID50 requires mathematical transformations of the data to calculate the final titer, using one of several methods such as: Spearman–Karber, Reed–Muench, Improved Karber , Weil, or Probit/logit regression. For plaque assay, the math is simpler: (# of plaques X dilution factor) divided by the volume of the inoculum added to cells in milliliters = plaque forming units per ml.

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