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BioRender Template
Degradation of Scaffolds for Tissue Engineering of Heart Valves Using Lipase
Creator
s
Description
Prepare a 0.05M potassium phosphate buffer solution (1). Titrate ph to 7.2 (2). Weigh out 9 mg of lipase (3). Add 45 ml buffer solution and the lipase to a tube (4). Incubate in a water bath for 30 minutes (5). Add 5 ml of lipase buffer solution and a scaffold to a tube (6). Incubation for 18 days with the given parameters (7). Dry the samples for 7 days (8). Subsequent examinations with balance, SEM and gravimetric porosity measurement.
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