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BioRender Template
Growing Norovirus in Human Intestinal Enteroids (HIEs)
Creator
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Description
This template was adapted from the original submission. Edits were made to enhance scientific accuracy, optimal usability and/or to meet industry-leading design standards for science communication.
This figure describes a protocol for growing human norovirus in human intestinal enteroids (HIEs). Briefly, 1) primary cells are collected from intestinal tissue and cultured as HIEs, which are 2) maintained weekly. 3) HIEs are trypsinized, strained, and used to seed monolayers. When monolayers are confluent, Wnt growth factor is removed in order to differentiate cells. 4) Monolayers are then infected in duplicate with norovirus, incubated to allow virus to bind, and washed. One monolayer is frozen and the second grown for 72 hours. Finally, norovirus RNA is extracted and analyzed using RT-qPCR to compare viral growth.
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