Schematic representation of the temperature change from 20°C to 25°C applied to adult flies for 5 days at each generation to induce a transient somatic knockdown of Piwi (Piwi-sKD) followed by constant maintenance at 20°C until the next generation. G0F100 corresponds to a sub-population of the initial G0 parental strain that was constantly kept at 20°C for 100 generations. During the successive Piwi-sKDs, three large populations corresponding to the offsprings of flies at generations 11, 31 and 73 (G11F1, G31F1, G73F1) were isolated and kept at 20°C. The offsprings of these isolated populations (G11F2, G31F2 and G73F2) were raised at 20°C and were sequenced using Nanopore technology.
