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Alternative processing of microRNAs
Description
This template shows alternative processing of microRNAs, comparing canonical Drosha and DGCR8 cleavage of a pri-miRNA hairpin to an alternative cleavage site that shifts the 5' end and produces an isomiR. It also shows Dicer1 processing into Ago-loaded miRNA and a target recognition panel comparing seed pairing of the canonical miRNA versus the 5' shifted isomiR, showing how each expands the pool of canonical and alternative mRNA targets.
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