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Annexin V-AF488 Apoptosis assay

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Rachid Boutoual

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Description: During early stages of apoptosis, there is a loss of membrane phospholipid asymmetry, leading to the exposure of phosphatidylserine on the cell surface. Annexin V, a protein with high affinity for PS residues, can detect this occurrence in the presence of Ca2+. Together with Propidium Iodide (PI), it can differentiate between healthy (Annexin−PI−), apoptotic Annexin+PI−), and necrotic (Annexin+PI+) cells, and be used for quantitative analysis of cell death.

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What does the Annexin V-AF488 Apoptosis assay template show?

During early stages of apoptosis, there is a loss of membrane phospholipid asymmetry, leading to the exposure of phosphatidylserine on the cell surface. Annexin V, a protein with high affinity for PS residues, can detect this occurrence in the presence of Ca2+. Together with Propidium Iodide (PI), it can differentiate between healthy (Annexin−PI−), apoptotic Annexin+PI−), and necrotic (Annexin+PI+) cells, and be used for quantitative analysis of cell death.

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