BioRender Template

Arrayed CRISPR screens

Created by

Dario Palmieri

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Description:

This template outlines a five-step workflow for arrayed CRISPR screens, starting with a Cas9-expressing cell line and a one-gRNA-per-well library mixed by high-throughput liquid handlers into a transduction plate. This is followed by high-throughput electroporation, incubation, and phenotypic analysis using high content imaging, flow cytometry, and live-cell growth analysis instruments.

Updated

July 2026

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