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Determining bacterial concentration using serial dilution

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Nicola Stonehouse

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Description: Schematic showing method of determining the concentration (CFU/mL) of an unknown bacterial sample using serial dilution. 1 mL of a sample of unknown concentration is serially diluted in 9 mL broth. These samples are then plated out onto appropriate media and incubated overnight. Following incubation colonies on plates are counted and used to determine CFU/mL of the original sample.

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What does the Determining bacterial concentration using serial dilution template show?

Schematic showing method of determining the concentration (CFU/mL) of an unknown bacterial sample using serial dilution. 1 mL of a sample of unknown concentration is serially diluted in 9 mL broth. These samples are then plated out onto appropriate media and incubated overnight. Following incubation colonies on plates are counted and used to determine CFU/mL of the original sample.

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