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Bacterial strain isolation and identification by 16S rRNA secuencing

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Cristina Fra

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Description: isolation of a single bacterial strain from a pool, growth analisis by periodicaly measuring optical density in spectrophotometer, those results are collected in a growth kinetic, followed by the extraction of bacterial DNA, PCR, and gel band extraction of the DNA that will be send to 16S rRNA secuencing, the data is analized to determine the taxonomic identification of each strain allowing to determine the phylogenetic similarities between them and other described strains

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isolation of a single bacterial strain from a pool, growth analisis by periodicaly measuring optical density in spectrophotometer, those results are collected in a growth kinetic, followed by the extraction of bacterial DNA, PCR, and gel band extraction of the DNA that will be send to 16S rRNA secuencing, the data is analized to determine the taxonomic identification of each strain allowing to determine the phylogenetic similarities between them and other described strains

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