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BioRender Template
BCA assay
Description
This template outlines the five steps of a BCA protein assay, starting with preparing the BCA reagent from reagents A, B, and C, then combining sample, reagent, and PBS in tubes showing increasing protein concentration. The samples are incubated for 60 minutes at 60 degrees Celsius, cooled to room temperature, and their absorbance is read at 562 nanometers on a plate reader after the copper-BCA complex forms a purple color.
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