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Bottom Up Plant proteomics
Description
This template outlines a six step plant proteomics workflow using the PreOmics iST kit, starting with heterologous protein expression via agroinfiltration in N. benthamiana leaves, followed by cryogrinding in liquid nitrogen and lysis with reduction and alkylation. The remaining steps show protein digestion, peptide purification on a cartridge, and final LC-MS/MS analysis for peptide identification.
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