BioRender Template
Caspase-3 Detection+DEVD Workflow
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Description:
The assay uses the fluorogenic substrate DEVD-AFC that is cleaved by active caspase-3, releasing a fluorescent product. The fluorescence intensity is directly proportional to caspase-3 activity and thus the level of apoptosis.
Updated
August 2026
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The assay uses the fluorogenic substrate DEVD-AFC that is cleaved by active caspase-3, releasing a fluorescent product. The fluorescence intensity is directly proportional to caspase-3 activity and thus the level of apoptosis.








