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Cloning by PCR (TA Cloning)
Description
This template outlines the TA cloning workflow, showing parallel preparation of a PCR-amplified insert with an added A-overhang and a linearized vector with an added T-overhang using terminal transferase or Taq polymerase. It shows the two pieces ligating together at the complementary A-T overhangs to form the final recombination vector.
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