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Clonogenic Survival Assay
Description
This template outlines the six steps of a clonogenic survival assay, from seeding cells at low density in a well plate and treating them with drug or radiation at different dosages, through incubating and monitoring colony growth over several weeks. The final steps show washing, fixing, and staining colonies with crystal violet dye, counting them, and plotting a survival curve comparing two treatments across dosage.
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