Colony PCR Protocol
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Description:
This template shows a four-step colony PCR protocol, beginning with picking bacterial colonies from a petri dish with a sterile pipette, noting that white colonies should be picked when using a LacZ reporter. The remaining steps show adding the colony to a PCR tube, running PCR with an extended 94C lysis step, and running a DNA gel to check for the expected PCR product.
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This template shows a four-step colony PCR protocol, beginning with picking bacterial colonies from a petri dish with a sterile pipette, noting that white colonies should be picked when using a LacZ reporter. The remaining steps show adding the colony to a PCR tube, running PCR with an extended 94C lysis step, and running a DNA gel to check for the expected PCR product.