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Competition for nuclear receptor protein sites between fluorescent probes and other small molecules
Creator
s
Description
1-anilinonaphtha-lene-8-sulfonic acid , 1,8-ANS is used as a fluorescent probe for fluorescence displacement detection. The fluorescent probe is non-fluorescent in an aqueous buffer but produces strong fluorescence once it binds to a hydrophobic environment (e.g., the LBD region of PPARγ). A decrease in fluorescence is expected if the test chemical replaces the probe in the hydrophobic protein binding pocket.
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