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BioRender Template
CRISPR Technology: Chromatin Imaging
Description
This template shows how CRISPR-based chromatin imaging uses a dCas9 protein guided by three different gRNAs, each carrying a distinct RNA hairpin, PP7, MS2, or boxB, that recruits a fluorescently tagged coat protein, GFP-MCP, BFP-MCP, or RFP-MCP. The three parallel panels illustrate how each hairpin-protein pair binds a specific genomic locus to enable multi-color imaging of chromatin.
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