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BioRender Template
CRISPR Technology: Gene Editing dCas9-FokI
Description
This template shows CRISPR dCas9-FokI gene editing technology, in which two catalytically dead Cas9 proteins, each guided by a separate gRNA and targeting a distinct PAM site, bring together a FokI nuclease dimer at the DNA target. This dimerization triggers FokI-mediated cleavage, producing a double-stranded break that is repaired via NHEJ or HDR.
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