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CRISPR Technology: Genomic Loci Purification
Description
This template shows a CRISPR-based genomic loci purification method, where dCas9 guided by an RNA scaffold with a biotin tag binds a specific DNA sequence alongside cohesin and cohesin-associated proteins. Streptavidin affinity purification of the biotin-tagged complex enables downstream proteomics, RNA-seq, and 3C-seq analysis of chromatin-associated proteins, RNA, and DNA.
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