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BioRender Template
CRISPR/Cas gene editing
Description
This template outlines four steps in CRISPR/Cas gene editing, starting with gRNA primer design based on a target DNA sequence and oligo synthesis. It continues through PCR amplification of the gRNA primers using template DNA and forward and reverse primers, and restriction digestion of the plasmid pIA33 with Msc1 and Not1 enzymes.
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