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CRISPRi
Description
This template shows a CRISPRi screening workflow using dCas9-KRAB expressing ventricular fibroblasts infected with sgRNA lentivirus targeting 10 to 15 guides per enhancer, arrayed into a 384-well plate. After antibiotic selection, RNA is isolated and converted to cDNA for gene expression analysis on a qPCR instrument.
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