BioRender Template

Cross-Linking & Immunoprecipitation (CLIP)-Seq Protocol

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Nick Delrose

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BioRender Team

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Description: This template outlines a ten-step CLIP-seq protocol for identifying RNA-binding protein binding sites, starting with in vivo UV cross-linking, cell lysis, and partial RNase digestion. It continues through immunoprecipitation, adapter ligation, SDS-PAGE purification, proteinase K digestion, reverse transcription, PCR sequencing, and bioinformatic peak-calling analysis.

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What does the Cross-Linking & Immunoprecipitation (CLIP)-Seq Protocol template show?

This template outlines a ten-step CLIP-seq protocol for identifying RNA-binding protein binding sites, starting with in vivo UV cross-linking, cell lysis, and partial RNase digestion. It continues through immunoprecipitation, adapter ligation, SDS-PAGE purification, proteinase K digestion, reverse transcription, PCR sequencing, and bioinformatic peak-calling analysis.

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