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Double-Selection Strategy vs. Single-Selection Strategy

An editable high resolution scientific image depicting Double-Selection Strategy vs. Single-Selection Strategy

Description

The figure depicts the double-selection and single-selection strategies for allelic exchange mutants. In the double-selection method (A), a two-step process with a counterselectable marker is employed, enabling the construction of defined unmarked mutants. The single-selection approach (B) involves introducing a selectable-counterselectable cassette into the target gene, resulting in its interruption. Subsequent allelic exchange within the mutated gene leads to the loss of the cassette.
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