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Endothelial Cell Permeability after Conditioned Medium Transfer
Description
This template outlines a three-step experiment measuring endothelial permeability, starting with treating HMC3 microglial cells with vehicle, Drug A, amyloid-beta, or Drug B, then clearing the conditioned medium by sequential centrifugation to remove cells, dead cells, and debris. The cleared medium is transferred onto HBMVEC brain endothelial cells in a transwell insert, and FITC-dextran leakage into the receiver well is measured to assess endothelial permeability.
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