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BioRender Template
Ex Vivo Activation of Mouse Spleen Leukocytes
Description
This template outlines a four step protocol for ex vivo activation of mouse spleen leukocytes, starting with spleen extraction and digestion into a single leukocyte suspension, followed by culture and activation using stimulants like LPS, anti-CD3/28, or ConA in a 3D culture plate. The final step covers analysis of the supernatant for cytokines, cell proliferation and viability, and activation markers.
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