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BioRender Template
Experimental Protocol for stable cell line generation
Description
This template outlines a five-stage timeline for generating a stable cell line, starting with transfection on day negative one and transfection analysis by day three using GFP expression and qPCR. It continues through culturing and antibiotic selection from day three to seventeen, colony isolation from day eighteen to thirty-two, and final verification of overexpression or knockdown by microscopy, qPCR, and western blotting.
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