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BioRender Template
Fluorescent Dye-Based Real Time PCR (qPCR) 4 Steps
Description
This template shows the four steps of fluorescent dye-based real-time qPCR: initiation with undetected fluorescence, denaturation at 95C that separates the DNA strands, and primer annealing at 60C. A fourth panel shows extension at 72C, where polymerase incorporates fluorescently labeled nucleotides.
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