BioRender Template

Gateway Cloning

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Science Design (BioRender)

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BioRender Team

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Description: This template shows the Gateway cloning method in two steps, a BP reaction that recombines a gene flanked by attB sites with a donor vector to generate an entry vector, and an LR reaction that transfers the gene from the entry vector into a destination vector to produce an expression vector. Both reactions release a toxic ccdB-containing byproduct that is excluded from the final product.

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BioRender Template FAQs

What does the Gateway Cloning template show?

This template shows the Gateway cloning method in two steps, a BP reaction that recombines a gene flanked by attB sites with a donor vector to generate an entry vector, and an LR reaction that transfers the gene from the entry vector into a destination vector to produce an expression vector. Both reactions release a toxic ccdB-containing byproduct that is excluded from the final product.

Is the Gateway Cloning template free to use?

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Can I edit or customize this template?

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

Is this template accepted for use in journals, theses, or grant applications?

Yes, BioRender icons and templates meet journal, grant, and copyright standards for publication, and many researchers cite BioRender directly in their figure legends.

How do I cite BioRender if I use this template?

Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

What is Gateway Cloning used for / who typically uses this template?

Researchers and scientists use this template for publications, funding proposals, grant applications, presentations, posters, and more.