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BioRender Template
Gelatin Zymography
Creator
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Description
Protocol workflow describing Gelatin Zymography, a technique that utilizes a modified SDS-polyacrylamide gel electrophoresis (PAGE) to assess the gelatinolytic activity of enzymes such as Matrix Metalloproteinases 2 and 9 (MMP-2 and MMP-9). The presence of bands shows hydrolytic activity influenced by conditions such as incubation time, protein quantity and protein quality. This method can be used to test gelatinase presence and activity in tissue/cell lysates and purified protein preps.
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