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Golden Gate cloning overview

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Baeli Spedding

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Description: Golden Gate cloning is a DNA assembly method that uses Type IIS restriction enzymes, including BsaI and BpiI, that can be used to assemble transcriptional units from standard modular parts (i.e. promoters, terminators, and coding sequences), and assemble multigene constructs from a combination of the transcriptional units made (based on Engler et al., 2014).

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What does the Golden Gate cloning overview template show?

Golden Gate cloning is a DNA assembly method that uses Type IIS restriction enzymes, including BsaI and BpiI, that can be used to assemble transcriptional units from standard modular parts (i.e. promoters, terminators, and coding sequences), and assemble multigene constructs from a combination of the transcriptional units made (based on Engler et al., 2014).

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