HiFi Sequence Read Generation
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Description:
This template shows the six steps of HiFi sequence read generation, starting with double stranded DNA that is made into SMRTbell libraries with hairpin adapters. Primers and DNA polymerase bind the circularized molecule, which is sequenced in repeated passes to generate multiple subreads that are trimmed and combined into a consensus HiFi read with over 99.9% accuracy.
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This template shows the six steps of HiFi sequence read generation, starting with double stranded DNA that is made into SMRTbell libraries with hairpin adapters. Primers and DNA polymerase bind the circularized molecule, which is sequenced in repeated passes to generate multiple subreads that are trimmed and combined into a consensus HiFi read with over 99.9% accuracy.