Histone Enrichment workflow
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Description:
This template shows a six-step histone extraction workflow, starting with nuclei enrichment using Triton X-100 buffer and acid extraction of histones, followed by acetone precipitation and a BCA assay to determine protein concentration. It continues through chemical derivitization of histones with propionylation and trypsin digestion, ending with nano LC-MS/MS analysis showing a resulting mass spectrum.
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This template shows a six-step histone extraction workflow, starting with nuclei enrichment using Triton X-100 buffer and acid extraction of histones, followed by acetone precipitation and a BCA assay to determine protein concentration. It continues through chemical derivitization of histones with propionylation and trypsin digestion, ending with nano LC-MS/MS analysis showing a resulting mass spectrum.