BioRender Template

HR and NHEJ reporter mechanisms for DNA double strand break repair.

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Riya Patra

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Description: This template shows a reporter construct with neomycin, GFP, and ISCI cut sites inside a cell membrane, where ISCI enzyme cleavage combined with an exogenous donor triggers repair by either non-homologous end joining or homologous recombination. NHEJ repair restores GFP expression alone, while HR repair with the donor inserts mCherry alongside GFP, producing GFP-positive or mCherry-positive cell populations that can be distinguished by fluorescence.

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BioRender Template FAQs

What does the HR and NHEJ reporter mechanisms for DNA double strand break repair. template show?

This template shows a reporter construct with neomycin, GFP, and ISCI cut sites inside a cell membrane, where ISCI enzyme cleavage combined with an exogenous donor triggers repair by either non-homologous end joining or homologous recombination. NHEJ repair restores GFP expression alone, while HR repair with the donor inserts mCherry alongside GFP, producing GFP-positive or mCherry-positive cell populations that can be distinguished by fluorescence.

Is the HR and NHEJ reporter mechanisms for DNA double strand break repair. template free to use?

Yes, you can customize this template with a free BioRender account (no credit card required). Paid plans unlock additional export formats, higher-resolution downloads, and publication permissions.

Can I edit or customize this template?

Yes, every element is fully editable. Icons, labels, colors, arrows, shapes and layout can all be changed to match your preferences or needs using BioRender's drag-and-drop editor. You can add or remove elements to fully customize your use case.

Is this template accepted for use in journals, theses, or grant applications?

Yes, BioRender icons and templates meet journal, grant, and copyright standards for publication, and many researchers cite BioRender directly in their figure legends.

How do I cite BioRender if I use this template?

Unlock publication permissions with a paid plan, and include 'Created with BioRender.com' in your figure legend, or cite BioRender directly per your journal's guidelines.

What is HR and NHEJ reporter mechanisms for DNA double strand break repair. used for / who typically uses this template?

Researchers and scientists use this template for publications, funding proposals, grant applications, presentations, posters, and more.