iMGL differentiation from iPSC
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Description:
This template shows the timeline for differentiating iPSCs into microglia-like cells (iMGLs) through an intermediate hematopoietic progenitor cell stage, spanning day 0 through day 28. It details the media changes and supplements used at each stage, such as HPC basal media, iMGL base media with MCSF, IL-34, and TGF-beta, and additional factors CX3CL1 and CD200 before the cells are ready for experiments.
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This template shows the timeline for differentiating iPSCs into microglia-like cells (iMGLs) through an intermediate hematopoietic progenitor cell stage, spanning day 0 through day 28. It details the media changes and supplements used at each stage, such as HPC basal media, iMGL base media with MCSF, IL-34, and TGF-beta, and additional factors CX3CL1 and CD200 before the cells are ready for experiments.