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BioRender Template
Immunofluorescence Assay with Negative Control
Creator
s
Description
This figure illustrates the steps of an immunofluorescence assay, comparing an experimental sample to a negative control. The experimental sample involves antigen detection using primary and fluorophore-labeled secondary antibodies, while the negative control omits the primary antibody to prevent specific antigen binding. Unbound secondary antibodies are washed off using PBS, ensuring specificity in fluorescence detection.
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