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BioRender Template
Isolating rat neonatal cardiac fibroblasts
Creator
s
Description
Rat pups 1-3 days old, isolating hearts after decapitation, overnight treatment with trypsin, followed by collagenase digestion 5 times, centrifuge to collect the cell palate, wash with HBSS and resuspended with media, grown on petri dish for 75 mins for the fibroblast to attach, media change after 75 mins and allowed to incubate for 48 hrs with fresh media.
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