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BioRender Template
Lifting iPSCs for Cell Passage
Creator
s
Description
The main goal of passaging iPS cells is to maintain their pluripotency, viability, and growth potential over successive generations.
One of the steps in the cell passage is lifting or detaching the cells from the base of the dish. iPSCs are typically dissociated using enzymatic methods. These enzymes break down cell-cell and cell-substrate interactions, facilitating the detachment of cells.
As a result, iPSCs begin to lift from the culture dish, becoming more rounded and less adherent.
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